The immunoblot provides highly specific information as to whether an antibody response is present against the pathogen being tested. The detection of IgM antibodies usually indicates a fresh infection. However, IgM antibodies can also persist over long periods of time in chronic cases. IgG antibodies occur at the earliest one to two weeks during an infection and represent an active or past infection. IgG antibodies are particularly present in chronic forms (or late forms) of Lyme disease and indicate activity in conjunction with clinical symptoms.
The immunoblot contains separated single antigens of the pathogens against which antibodies (IgG and IgM) are bound and stained in the patient’s blood on a blot strip. The longer an infection persists, the more the body learns about the antigens of the pathogen and this is reflected in the number of detected bands in the ‘western blot’. The ratio of IgM to IgG antibodies can also help to understand a patient’s congestion (current early phase, acute late phase, chronic or past infection). The western blot also contains a surface marker called VlsE, which has the highest sensitivity for pathogen detection.
1 x serum tube (centrifuge after blood collection)
Analytical test duration:
Note: Taking into account the pre- and post-analytical work processes, the report of findings is usually prepared in approx. 1 week).
Immunoblot accounting according to GOÄ:
2 x number A4409 (IgM and IgG antibodies) Factor 1.15